MicroRNA对子宫颈癌前病变的诊断价值的研究

摘要

目的 研究microRNA对子宫颈癌前病变的诊断价值.方法 选择宫颈上皮内瘤变患者作为观察组(120例)、宫颈良性病变患者作为对照组(100例),采用Trizol法抽提两组宫颈样本组织的总mRNA、Stem-loop RT PCR的方法合成cDNA第一链、荧光定量PCR的方法检测目的 microRNA的含量,进行比较分析.结果 观察组miR21、miR221和miR222含量明显高于对照组,且呈现出CINⅠ级组＞CINⅡ级组＞CINⅢ级组,miR-10b、miR-9、miR15a、miR16含量明显低于对照组,且呈现出CINⅠ级组＜CINⅡ级组＜CINⅢ级组.结论 良性病变与癌前病变的宫颈组织中microRNA存在显著差异,对子宫颈癌前病变具有一定的诊断价值.%Objective To study the diagnostic significance of microRNA in diagnosis of uterine cervical precancerous lesions. Methods One hundred and twenty patients with cervical intraepithelial neoplasia( CIN )were allocated to observation group and 100 patients with cervical benign lesions were allocated to control group. Total mRNA in tissue of cervical cancer was extracted by Trizol method, first chain cDNA was synthesized by Stem - loop RT PCR and gene content was detected by fluorescent quantitative PCR. Results The contents of miR21, miR221 and miR222 in patients of observation group were significantly higher than those of patients in control group, and they presented as CIN I > CIN II > CIN Ⅲ, and the contents of miR - 10b, miR -9, miR15a and miR16 were significantly lower than those of patients in control group and they were presented asCIN I < CIN Ⅱ < CIN Ⅲ. Conclusion There is significant difference in contents of microRNA between benign and precancerous lesions of cervix tissue, hence it is helpful in diagnosis of precancerous lesions in cervix tissue.