Sirt1在神经胶质瘤及瘤旁组织中的表达差异研究

摘要

Objective To explore the changes in gene Sirt1 expressions in tissues of human glioma and para - glioma. Methods A 44 000 gene cDNA microarray(Affymetrix)was used to examine gene expressions in the tissues of human glioma and para - glioma. The Sirt1 genes were identified and subjected to real - time PCR analysis. Results The expression of Sirt1 in tissues of glioma was extremely lower than that in tissues of para - glioma. The results of real - time PCR were consistent with those of genechip analysis. Conclusion The expressions of Sirt1 were obviously different between tissues of glioma and para - glioma. Sirt1 may be associated with carcinogenesis of glioma.%目的研究神经胶质瘤及瘤旁组织中的沉默信息调节因子1（Sirt1）基因的表达差异。方法应用基因芯片技术检测30例Sirt1基因在神经胶质瘤及瘤旁组织的表达差异，随后应用RT-PCR及免疫组织化学实验验证并分析Sirt1基因的表达差异。结果基因芯片筛选出存在差异表达的基因，其中Sirt1基因在神经胶质瘤中的表达较瘤旁组织中的表达明显下降，RT-PCR实验证实与基因芯片结果一致，免疫组织化学实验结果与基因芯片、RT-PCR结果一致。结论 Sirt1在神经胶质瘤及瘤旁组织中的表达存在明显差异，Sirt1基因的表达可能与神经胶质瘤的发生发展密切相关。