黄芪联合干扰素α对 CCl4诱导的大鼠肝纤维化的保护作用

摘要

Objective To explore the intervention effects of Huangqi(Astragalus)combined with IFN - α on the liver fibrosis in rats in-duced by the carbon tetrachloride(CCl4 )and its possible mechanism. Methods Liver fibrosis was induced in rats by intraperitoneal injection of CCl4 for 6 w. SD rats were randomly divided into five groups:normal control group,model group,Huangqi group,IFN - α group and Huangqi +IFN - α group. Then levels of HA,LN,PC - III and IV - C were detected in different groups. The levels of were detected at the same time by u-sing Jamall method. HYP TIMP - 1 and MMP - 1 levels were detected at the same time by using ELISA. Results Compared with model group, levels of HA,LN,PC - III and IV - C in the treatment groups were obviously decreased( P ﹤ 0. 01). Compared with Huangqi group and IFN -α group,the levels of HA and IV - C decreased in combination group( P ﹤ 0. 05). The levels of TIMP - 1 decreased significantly,and the level of MMP - 1 in increased significantly in combination group compared with those in model group( P ﹤ 0. 05). Conclusion Huangqi combined with IFN - α have protective effect of liver fibrosis caused by CCl4 ,and the mechanism may be related the adjustment of MMP - 1 and TIMP - 1 expression.%目的：观察黄芪联合干扰素α对四氯化碳（CCl4）诱导的大鼠纤维化病变的干预效果及可能的作用机制。方法采用诱导 CCl4肝纤维化，造模6周，SD 大鼠随机分为5组：正常对照组、模型组、黄芪组、干扰素α组和黄芪+干扰素α联合治疗组。采用放免法检测大鼠血清透明质酸（HA）、层黏连蛋白（LN）、Ⅲ型前胶原（PC - III）、Ⅳ型胶原（IV- C）；同时使用 Jamall 法测定肝组织羟脯氨酸（HYP）的水平；采用 ELISA 检测大鼠肝组织中基质金属蛋白酶组织抑制剂-1（MMP -1）和基质金属蛋白酶抑制因子-1（TIMP -1）含量的变化。结果黄芪和干扰素均能显著降低肝纤维化大鼠血清中 HA、LN、PC - III 和 IV - C 的含量（ P ﹤0.01）；而且与单一药物治疗组相比，联合药物组治疗后大鼠 HA 和IV - C 的表达水平降低更明显（ P ﹤0.05）。黄芪和干扰素联合用药可以导致肝纤维化大鼠 TIMP -1表达水平的降低和 MMP -1表达水平的增高（ P ﹤0.05）。结论黄芪联合干扰素α对 CCl4诱导的大鼠肝纤维化具有保护作用，这种保护作用的产生可能与调节肝组织中 TIMP -1、MMP -1表达水平有关。