四氧化二氮吸入中毒致小鼠急性肺水肿药物干预实验研究

摘要

Objective To establish the mice model of acute pulmonary edema and investigate the therapeutic effect of the compound ami-no acids and vitamin B6. Methods Ten ICR mice(23˜25 g)were randomly divided into control group,poisoned group,and treatment group (n=8). The mice in poisoned group and treatment group were put in the 120 L exposed cabinet to establish N2O4 inhalation induced acute pulmo-nary edema model. Blood was obtained 48 h after the exposure. The mice in treatment group received composite amino acids and vitamin B6 for the treatment. ANP,SP-D,TGF-β1 and TNF-αwere detected by ELISA. Results There were significant differences in ANP,SP-D,TGF-β1 and TNF-α levels between poisoned group and control group( P <0. 05),and there were significant differences in ANP,SP-D and TNF-α levels between the treatment group and the poisoned group( P <0. 05). Conclusion N2 O4 inhalation poisoning can lead to pulmonary ede-ma. Mornitoring the changes of serum ANP,SP-D,TGF-β1 and TNF-αis of clinical significance for the study of the mechanism of N2 O4 in-halation poisoning. The treatment of composite amino acids in combination with vitamin B6 can significantly improve serum ANP,SP-D and TNF-α levels in N2 O4 inhalation poisoning animal. The study has certain reference value for the treatment of patients with this type of poisoning.%目的制作四氧化二氮（ N2 O4）吸入中毒致小鼠肺水肿动物模型，应用复合氨基酸加维生素B6予以救治，研究药物干预效果。方法实验动物为ICR小鼠，体重23˜25 g，随机分为对照组、中毒组和治疗组三组（ n=8）。将动物置于120 L染毒柜制作N2 O4吸入中毒致肺损伤动物模型，于48 h采用眼球摘除法取血，分离血清-70℃保存，酶联免疫分析（ELISA）法测定心钠素（ANP）和肺表面活性物质相关蛋白D（SP-D）、转化生长因子β1（TGF-β1）、肿瘤坏死因子-α（TNF-α）含量。结果中毒组与对照组之间ANP、SP-D、TGF-β1和TNF-α水平差异明显（ P ＜0.05）；治疗组与中毒组之间ANP、SP-D和TNF-α水平差异明显（ P ＜0.05），TGF-β1水平则无显著差异。结论 N2 O4吸入中毒可导致肺水肿，监测血清ANP、SP-D、TGF-β1和TNF-α的变化对研究N2 O4吸入中毒机制具有临床意义。采用复合氨基酸联用维生素B6能明显改善N2 O4吸入中毒动物血清ANP、SP-D和TNF-α代谢水平，促进病情转归，对于救治此类中毒患者具有一定参考价值。