七氟醚抑制培养鼠迷走传人神经元5-羟色胺3受体调控的电流

摘要

Objective and Methods:To assess the effects of 0.7, 1.4mM sevoflurane on 5-hydroxytryptamine3 receptors (5HT3R)-mediated current induced by bath application of 30μM 2-methy1-5-hydroxytryptamine, a selective 5-HT3R atagonist in rat nodose ganglia neurones maintained in primary culture and in comparison with those of both halothane and isoflurane. The whole-cell patch clamp recordings was applied. Results: The peak amplitude of transient inward current evoked by 30μM 2-methyl-5-hydroxytryptamine decreased to 77±12 % aand 64±9 % by 0.7 and 1.4mM sevoflurane respectively. In contrast, both halothane and isoflurane enhanced the transient inward current. Conclusion:Sevoflurane,at clinically anesthetic concentration, inhibits 5-HT3R-gated transient inward current evoked in rat vagal afferent neurones.%目的与方法：采用膜片钳全细胞记录技术，观察0．7、1．4mM七氟醚对培养鼠结状神经节细胞30μM二甲5-羟色胺引发的瞬间内向电流影响，并与氟烷、异氟醚作比较。结果：0．7、1．4mM七氟醚分别抑制5-羟色胺3受体调控的瞬间内向电流的峰值至对照值的77％±12％和64％±9％。但氟烷、异氟醚均增强该电流。结论：结果提示临床麻醉浓度的七氟醚抑制鼠迷走传入神经元5-羟色胺3受体调控的瞬间内向电流。