针刺对慢性应激抑郁大鼠血清和脑炎性细胞因子的影响

摘要

Objective:To observe the effect of acupuncture on inflammatory cytokines response in serum and brain regions of depression .Methods:The chronic unpredictable stress ( CUS ) rat model with depression was employed in this study .The SD rats were randomly divided into four groups: the normal control, CUS, acu-puncture and fluoxetine .The contents of pro -inflammatory cytokines interleukin -1β( IL-1β) , IL-6 and tumor necrosis factor -α( TNF-α) were detected by ELISA .Results:Compared with control group , the con-tents of IL-1βin serum, hippocampus and frontal cortex in model group were significantly increased ( P<0.01 );the contents of IL -6 in serum , hippocampus and frontal cortex were significantly increased ( P <0.01 );the contents of TNF-αin serum, hippocampus and frontal cortex were significantly increased ( P<0.01 ) .Acupuncture significantly decreased the contents of IL -1β, IL-6 and TNF-αin the serum , hippo-campus and frontal cortex of the model group ( P<0 .01 ) .Conclusion:Acupuncture treatment could significant-ly reduce the inflammatory cytokines in serum and brain , and alleviate inflammatory response , which might me-diate the antidepression by acupuncture .%目的：探讨针刺对抑郁症外周和中枢炎性细胞因子的调节作用。方法：采用慢性不可预知的应激方法建立抑郁症大鼠模型。将SD大鼠随机分为正常组、模型组、针刺组和氟西汀组。采用酶联免疫吸附法（ ELISA ）检测炎性细胞因子白细胞介素-1β（ IL-1β）、白细胞介素-6（ IL-6）和肿瘤坏死因子-α（ TNF-α）的含量。结果：与正常组比较，模型组大鼠血清、海马、额叶皮层IL-1β含量均显著升高（P＜0．01），血清、海马、额叶皮层IL-6含量均显著升高（P＜0．01），血清、海马、额叶皮层TNF-α含量均显著升高（ P＜0．01）。针刺治疗可以显著降低模型大鼠血清、海马和额叶皮层IL-1β、IL-6和TNF-α含量（ P＜0．01）。结论：针刺可以抑制炎性细胞因子，缓解抑郁症外周和中枢炎症反应，这可能是针刺发挥抗抑郁作用的机制之一。