慢性肝病患者血清HBV-DNA定量检测及临床应用

摘要

Objective　To investigate the relationship among the content of serum HBV-DNA and the different degree of chronic liver damage,and the marks of hepatitis B virus and to explore its value in the evaluation of therapy.Method　Fluorescence quantitative PCR was used to measure the concentration of sera HBV-DNA in 113 patients infected by HBV.Results　The serum HBV-DNA in mild chronic hepatitis group was the highest.In the patients with aggravating liver damage,the serum HBV-DNA concentration was decreased gradually.It was indicated that serum HBV-DNA concentration in the patients with HBeAg positive was significantly higher than that of HBeAg negative.However,there were no evident relationship among HBV-DNA concentration,ALT,AST activities and different Child's classification of cirrhosis.The patients with lower serum HBV-DNA concentration before treatment had a better outcome.The therapeutical effects were correlated with the serum of viral DNA concentrations.Conclusions　The quantitative detection of serum HBV-DNA has instructive value in understanding the relationship among the clinical status of chronic hepatic disease,the duplication level of hepatitis virus and evaluating therapy.%目的探讨乙肝病毒(HBV)DNA含量与慢性肝病临床病程和乙肝标志物的关系及其在治疗疗效观察中的应用价值。方法采用TagMan荧光标记探针技术检测113例各型慢性肝病患者血清HBV-DNA含量。结果以慢性乙肝轻型HBV-DNA含量最高，随肝损害加重，HBV-DNA含量逐渐下降。血清HBV-DNA含量在HBeAg阳性组明显高于HBeAg阴性组，而与ALT、AST及肝硬化不同Child分级无相关性。治疗前HBV-DNA含量低者疗效好，且不同疗效HBV-DNA含量变化不同。结论定量PCR检测血清HBV-DNA含量对了解慢性乙肝患者病毒复制情况和临床病程的关系及治疗评价有一定价值。