传染性单核细胞增多症患儿CD3+CD4-CD8-T细胞与T淋巴细胞亚群变化情况及临床意义

摘要

Objective To study on the changes of the DNT cells and T lymphocyte subtype in children with infectious mononucleosis (IM) and its clinical significance.Methods The flow cytometry (FCM) was used to detected the DNT cells and other T lymphocyte subtype in 48 cases of IM.Results The study showed that DNT cells( 9.39 ± 4.89 )% were greatly increased in comparison with normal controls (NC) (4.26 ± 1.68)% ( P ＜0.01 ).CD4 cells(21.45 ±9.87)% were decreased ( P ＜0.01 ) and CD8 cells increased in comparison with NC (32.43 ± 5.07) % ( P ＜ 0.01 ).Conclusion DNT cells and T lymphocyte subtype can be used to evaluate the immune function of children with infectious mononucleosis (IM) and provide guidance for adoptive immunotherapy.%目的 研究传染性单核细胞增多症(IM)患儿外周血CD3+CD4-CD8-T细胞(DNT细胞)及T淋巴细胞亚群变化及其临床意义.方法 采用免疫荧光流式细胞技术检测了48例IM患儿及40例正常儿童外周血DNT细胞与T淋巴细胞亚群CD3+T细胞、CD3+CD4+T细胞及CD3+CD8+T细胞水平.结果 IM患儿DNT细胞百分比(9.39±4.89)%高于正常对照组(4.26±1.68)%(P＜0.01),CD3+CD4+T细胞百分比(21.45±9.87)%明显低于对照组(32.43±5.07)%(P＜0.01);而CD3+T细胞及CD3+CD8+T细胞百分比均明显高于对照组(P＜0.01).结论 IM患儿DNT细胞升高及T淋巴细胞亚群比例改变,提示IM患儿存在免疫功能异常,为IM患儿临床免疫治疗提供理论依据.