Radix Ophiopogon,a kind of traditional Chinese medicine,is the root of Lili-aceae Ophiopogon j aponicus (Thunb.)Ker Gaw l.Modern research demonstrates that Ophiopogon j aponicas can be used as a folk food medicine in free radical scavenging, reducing blood sugar,sedative hypnotic and anti-myocardial ischemia and arrhythmia, and so on.The major chemical compounds of this plant are mainly homoisoflavonoids, steroidal saponins and polysaccharides. According to the relevant reports, the homoisoflavonoids is a kind of specific compounds in Ophiopogon j aponicus .However, due to the large number of different types of homoisoflavonoids in the herbal medicine, the structures are too similar to be distinguished from each other.Meanwhile,the differences of their relative contents in Ophiopogon j aponicas are great.In this paper, the rapid separation ability of UPLC-LTQ-Orbitrap and the higher energy collision induced dissociation mode (HCD mode)were used to acquire high resolution fragment ion information and studied on the fragmentation patterns of homoisoflavonoids.The chromatographic separation of Ophiopogon j aponicus extract was used Waters Acuqity UPLCTM BEH C18 column,mobile phase was 0.05% formic acid in water and acetoni-trile system,and the chromatographic conditions for the rapid separation were opti-mized.To avoid interference with other components and obtain the tandem mass spec-trometry high content and low content data,a method based on parent ions detection list in the negative ion mode detection was established.Furthermore,the established meth-od was helpful in investigating the dissociation pathways of homoisoflavonoids in differ-ent HCD cleavage energy and confirming the characteristic fragment ions for the identifi-cation.Meanwhile,68 constituents attributed to homoisoflavonoids in Ophiopogon j a-ponicas are finally identified,including 26 homoisoflavonoids,31 homoisoflavanones, and 1 1 homoisoflavonoids glycosides.The results indicate that the HCD model can pro-vide abundant and stable information of fragment ions,which can be adopted to quickly and accurately clarify the homoisoflavonoids in complex system.This study can provide an effective analytical method to explore the material basis of sequential constituents in traditional Chinese medicines.%应用 UPLC-LTQ-Orbitrap 法快速鉴定麦冬药材中高异黄酮类化合物,考察不同高能诱导裂解(HCD)能量下高异黄酮的裂解情况,优选特征碎片离子对进行鉴定,并探索麦冬中高异黄酮类成分的裂解规律。应用该方法在麦冬提取物中鉴定出68个高异黄酮类成分,其中包括26个高异黄酮成分,31个高异黄烷酮成分,11个高异黄酮糖苷类成分。结果表明,HCD 模式能够提供丰富稳定的裂解碎片离子信息,结合 UPLC-LTQ-Orbitrap 技术能够快速准确地鉴定复杂组分中高异黄酮类成分,可为中药系列成分研究提供有价值的方法参考。
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