本文用裸鼠作为实验动物对化学刺激性物质导致角质层脂态的变化进行超微结构的研究.裸鼠表皮经丙酮和氯化苯甲烃铵处理后,样品用四氧化钌固定.超微结构观察表明由于丙酮和氯化苯甲烃铵的刺激作用导致角质层脂质形态的变化、角质层脂质的抽提以及在角质细胞之间形成腔隙.结果表明四氧化钌对角质层脂质有良好的固定作用,并清晰地展示出丙酮和氯化苯甲烃铵对角质层脂质的影响.%To gain a better understanding of the interaction of chemically different irritants with the stratum corneum lipids, the morphological changes of the stratum corneum lipids resulting from the chemical irritants in murine epidermis were investigated. Node mice were treated topically with either acetone or 2% benzalkonium chloride, the visualization of the stratum corneum was carried out using a special fixation method utilizing 0.25% ruthenium tetroxide as the post-fixative. Ultrastructural observation demonstrated obvious distortions within the intercellular domains of the stratum corneum, including disorganization of lipid bilayers, lipid extraction, and lacunae formation. The results of this study indicated that ruthenium tetroxide is a preferable post-fixative for the study of the stratum corneum lipids, and provide evidence that different irritants induce distinct alterations within the stratum corneum to reflect the specific interaction with the stratum corneum lipids.
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