HB-EGF对系膜细胞己糖激酶活性的调节

摘要

Objective: Our aim was to study the concentrations and regulatory characteristics of heparin-binding epidermal growth factor-like factor (HB-EGF) and its functional consequences in inducing the activities of hexokinases (HKs) and net Glc utilization. Methods: The SV40MES13 cell line was used as cell model for this experiment to measure the total activity of Hks by using a standard G6PDH-coupled assay. The net Glc utilization was measured indirectly as Glc disappearance from the medium using the standard Glc oxidase-coupled reaction(Trinder). Results: The HB-EGF induced HKs activity in time- and dose-dependent manners and increased the net Glc utilization by SV40MES13 cells. Conclusion: The HB-EGF plays an important role in pathogenesis of diabetic nephropathy by inducing total HKs activity specifically and increases the net Glc utilization in mesangial cells.%目的：探讨肝素结合性表皮生长因子（heparin-binding epidermal growth factor-like growth factor, HB-EGF）对肾系膜细胞己糖激酶（hexokinases，HKs）的调节及其对葡萄糖净利用的影响。方法：以SV40MES13系膜细胞株作为细胞模型，用6-磷酸葡萄糖脱氢酶偶联比色法检测系膜细胞HKs活性，并利用Trander氧化法检测系膜细胞葡萄糖的净利用。结果：HB-EGF以时间和剂量依赖形式诱导了系膜细胞HKs活性，并增加了系膜细胞对葡萄糖的净利用。结论：HB-EGF通过增加HKs活性，使系膜细胞内葡萄糖流量增加，参与了糖尿病肾病的发生和发展。