替米沙坦对胰岛素抵抗大鼠肾脏脂联素表达的影响

摘要

Objective To investigate the influences of telmiaartan on renal expression of adiponectin (APN) in insulin resistance rats. Methods Insulin resistance rat model was induced by long-term high-fat feeding and was assessed by euglycemic-hyperinsulinemia clamp technique. The morphological changes of the kidney lissue were observed by the light and electronic microscopy.Glomerular APN mRNA ex-pression and protein synthesis were evaluated by the reveree transcription polymerase chain reaction and immunohistochemical staining re-spectively. Results Both APN mRNA and APN protein were lower in insulin resistance rats than those in control rats (P < 0.05). More-over, the main features of the renal parenchyma were observed in insulin resistance rats. The expression levels of APN mRNA and APN pro-tein were increased after the treatment with lelmisartan (P <0.05). The renal damage was significantly reduced as well. Conclusion The up-regulation of APN expression induced by telmisartan might prevent the renal damage of insulin resistance.%目的 探讨替米沙坦对胰岛素抵抗大鼠肾脏脂联素(APN)表达的影响.方法 采用高脂饲料喂养10周建立胰岛素抵抗大鼠模型,并用正常血糖一高血浆胰岛素钳夹技术评估.光镜及电镜行肾脏形态学检查,逆转录聚合酶链反应方法测定APNmRNA表达,免疫组化染色观察APN蛋白水平变化.结果 胰岛素抵抗大鼠肾脏APN mRNA和蛋白表达明显低于正常对照组(P均<0.05),同时大鼠肾脏出现病理变化;替米沙坦干预后,肾组织APN mRNA和蛋白表达较胰岛素抵抗组升高(P均<0.05),同时肾脏病理变化也得到明显改善.结论 替米沙坦可能通过调节肾脏APN的表达,起到保护胰岛素抵抗相关的肾脏损伤的作用.