目的 观察谷氨酸N-甲基-D-天冬氨酸(NMDA)受体拮抗剂5-甲基二氢丙环庚烯亚胺马来酸(MK-801)、α-氨基3-羟基5-甲基4-异恶唑丙酸/海人藻氨酸(AMPA/Kainate)受体拮抗剂6-氰基-7-硝基喹喔啉-2,3-二酮(CNQX)对尾末端截断后小鼠血清褪黑素(MLT)含量变化的影响,为进一步研究截肢后中枢的可塑性变化提供依据.方法 用ELISA法测定尾末端2.5 cm截断后2h小鼠血清MLT含量,并观察静脉注射MK-801、CNQX对断尾后小鼠血清MLT含量变化的影响.结果 与对照组比较,断尾后2h小鼠血清MLT含量降低；MK-801、CNQX分别拮抗断尾引起的小鼠血清MLT含量的降低.结论 断尾后2h小鼠血清MLT含量降低；NMDA受体、AMPA/Kainate受体参与断尾引起小鼠血清MLT含量降低的过程.%Objective To study the effect of glutamic acid receptor antagonist, MK-801 or CNQX on the change of serum melatonin contents of mice after amputating extremity of the tail, which could provide new scientific experimental data for further investigating plastic changes in central nervous system after amputation. Methods The distal 2.5 cm length of the mice tail was removed as an experiment model of amputation. The blood of the mice at 2 h after the amputation was obtained by cutting the mice heads. FLISA method was used to measure the serum melatonin contents of the mice. MK-801 or CNQX was injected via the caudalis vein. Results Compared with serum melatonin contents of the mice of control groups, serum melatonin content of the mice at 2 h after the amputation was significantly decreased (P<0.05).The intravenous injection of MK-801 or CNQX antagonized the decrease in the mice's serum melatonin contents at 2 h after the amputation. Conclusion The results indicate that serum melatonin content of the mice at 2 h after amputating extremity of the mice tail is significantly decreased. The NMDA receptor and AMPA/Kainate receptor are involved in the processes of decrease in serum melatonin contents induced by amputating extremity of the mice tail.
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