PCR-SSCP技术分析线粒体DNA多态性及其法医学应用

摘要

Objective To study the application of SSCP technology in the mitochondrial DNA analysis.Methods The highly variable region 16106-16297 in D-loop was amplified by PCR.150 unrelated Liaoning Han individuals,10 normal tissues and 5 formalin-fixed,paraffin-embedded tissues were investigated and analyzed by SSCP.Results In 150 unrelated Liaoning Han individuals,9 haplotypes were found by PCR-SSCP,DP=0.881.The 5 paraffin-embedded tissues displayed alleles different from those of corresponding normal tissue.Conclusion PCR-SSCP technology has a higher ability of mitochondrial DNA identification and is suitable for tests of degraded samples.%目的 探讨SSCP法在线粒体DNA分析中的应用及其在法医实际检案中的意义.方法 利用PCR方法直接扩增D-LOOP区的HVI16106-16297区域192 bp片段作为目的片段,进行SSCP分型.检验150例随机个体、10例正常组织和5例甲醛固定石蜡包埋的组织.结果 在150例随机个体中,SSCP检测发现9种单倍型,DP值为0.881.在10例正常组织中,各个组织的SSCP带型完全相同.5例包埋组织的SSCP分型与对照组织一致.结论 SSCP技术分析线粒体DNA有较高的鉴别能力,适用于陈旧降解检材的检验.