生物净化烟气NO_x填料塔生物膜的细菌优势种群

摘要

利用免培养的变性梯度凝胶电泳（PCR-DGGE）这一分子生物学手段,研究了脱氮塔中生物膜细菌的种群结构。发现其优势种群分属于：硝化杆菌属Nitrobacter sp.、热单胞菌属Ther-momonas sp.、不动杆菌属Acinetobacter sp.、绿弯菌属Chloroflexus sp.。实验结果表明：将NOx氧化为NO3-的功能菌主要是亚硝酸盐氧化菌Nitrobacter sp.;起硝化作用的自养菌与异氧菌共生在填料塔生物膜中。异养菌除解除了自养菌的有机产物抑制外,还发挥了反硝化作用,生成了易挥发的N2,导致了脱氮系统的N元素损失,同时也产生了碱性物质。在分子水平从种群组成的角度揭示了生物净化烟气NOx的部分机理。%Bacterial population composition of biofilms in biotrickling filter was analyzed with one culture-independent method by denaturing gradient gel electrophoresis（PCR-DGGE）.It is found that Nitrobacter sp.,Acinetobacter sp.,Thermomonas sp.and Chloroflexus sp.are the dominant species of the biofilms.Nitrobacteria are the primary bacteria which oxidize NOx into NO-3.Autotrophic bacteria that act on the nitrification are symbiotic with heterotrophic microbes.The heterotrophic bacteria besides can relieve organic production inhibition against autotrophic bacteria,but also make the denitrification by generating N2 etc.and simultaneously produced alkaline production.The part mechanism of bio-purification NOx in the coal-fired flue gas in the view of bacterial community at molecular level is disclosed.