软肝冲剂抗大鼠肝纤维化的实验研究

摘要

Objective: To explore the therapeutic effects of Ruanganchongji on rat liver ifbrosis model.Methods: 50 male Wistar rats were randomly divided into normal control group, model group, Ruanganchongji low dose group (4g/kg/d), Ruanganchongji high dose group (8g/kg/d) and Biejiaruanganpian group (0.8g/kg/d), with 10 rats in each group. The rat liver ifbrosis model was established by lavaging 40% carbon tetrachloride and 40% alcohol; then the rats in each drug group were lavaged with corresponding drugs for 8-week. The liver function, liver ifbrosis indexes (laminin and hyaluronic acid) and hydroxyproline content in liver were respectively detected.Results:Ruanganchongji could improve the liver function of liver ifbrosis rat model; reduce the content of laminin, hyaluronic acid, and hydroxyproline content in liver obviously. Moreover, the effects of Ruanganchongji high dose were better than Ruanganchongji low dose and Biejiaruanganpian.Conclusions:Ruanganchongji has better therapeutic effects on liver ifbrosis model rats, but the mechanisms need further study.%目的：探讨软肝冲剂对大鼠肝纤维化模型的治疗作用。方法：50只雄性Wistar大鼠随机分为正常对照组、模型组、软肝冲剂低剂量组(4g/kg/d)、软肝冲剂高剂量组(8g/kg/d)和鳖甲软肝片组(0.8g/kg/d)，每组10只大鼠。40%四氯化碳和40%酒精灌胃制备大鼠肝纤维化模型，各用药组大鼠给予相应药物灌胃8周。分别检测各组大鼠的肝功能，肝纤维化指标层粘连蛋白(LN)、透明质酸(HA)和肝组织羟脯氨酸(HYP)的含量。结果：软肝冲剂可明显改善肝纤维化大鼠模型的肝功能，降低LN、HA和肝组织HYP的含量；并且，软肝冲剂高剂量的效果明显优于软肝冲剂低剂量和鳖甲软肝片。结论：软肝冲剂对肝纤维化大鼠模型具有较好的治疗效果，但具体机制尚待进一步研究。