黄芩药材不同取样部位指纹图谱研究

摘要

Objective:To investigate the changes of 10 kinds flavonoids contents in different sampling parts of Scutellaria baicalensis. Methods:TC-C18 chromatographic column was used in this study:gradient elution with acetonitrile and 0.1%glacial acetic acid as mobile phase, detection wavelength was 276 nm, flow rate was 1.0ml/min, column temperature was 25℃. The data was analyzed by area normalization method. Results:The content ratio of 10 kinds lfavonoids was different in different sampling parts of third-year Scutellaria baicalensis in fall:from top to bottom, glycoside gradually decreased, while aglycone gradually increased. Conclusions:The lfavonoids contents are different in different sampling parts of Scutellaria baicalensis, so researchers should ensure the scientiifc nature of sampling.%目的：：考察黄芩不同取样部位10种黄酮类成分含量的变化。方法：使用TC-C18色谱柱(4.6mm×250mm,5µm, Agilent)，流动相：乙腈与0.1%冰醋酸溶液进行梯度洗脱，检测波长276nm，流速为1.0ml/min，柱温25℃，以面积归一化法对数据进行分析。结果：秋季三年生黄芩不同取样部位10种黄酮类成分的含量比例各不相同，苷类成分含量从上到下越来越低、苷元类成分含量从上到下越来越高。结论：黄芩不同取样部位黄酮类成分的含量不同，应注意保证取样的科学性。