利用Langmuir-Blodgett技术和sol-gel法在氧化铟锡(ITO)电极上构建二氧化硅(SiO2)球腔微电极阵列.在此球腔微电极阵列上电沉积普鲁士蓝膜(PB),用于过氧化氢检测,并采用滴涂法将葡萄糖氧化酶(GOD)直接固定于PB/ SiO2球腔微电极阵列上制得葡萄糖传感器(酶电极),酶电极对葡萄糖电流响应结果表明:葡萄糖浓度在4.7×10-6 mol/L~3.8×10-3 mol/L范围内呈线性关系,其检出限为1.35×10-6 mol/ L,该酶电极不受抗坏血酸、尿酸等电活性物质的干扰,稳定性较好.% The silicon dioxide (SiO2) cavities microelectrode array was fabricated on the indium tin oxide (ITO) electrode, which was constructed by the Langmuir-Blodgett (LB) technology and sol-gel technology. The Prus⁃sian Blue (PB) film was fabricated on the SiO2 cavities array by electrodeposition technique, which was used to construct electrochemical sensor for the detection of hydrogen dioxide (H2O2). A glucose biosensor was obtained by drop-coating glucose oxidase (GOD) in PB/SiO2 cavities array /ITO electrode directly .The resulting biosensor was highly sensitive to glucose with a linear calibration plot in the concentration range of 4.7×10-6-3.8×10-3 mol/L glucose, the detection limit 1.35×10-6 mol/ L glucose (S/N=3). It is free of interference by ascorbic acid, uric ac⁃id and other active substances .and also have good stability.
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