生物功能化AgInS2量子点的制备及其生物应用

摘要

为制备水相、低毒、生物兼容性较好的三元量子点,通过水相合成法制备三元银铟硫(AgInS2)量子点,并用叶酸(FA)对其进行修饰,利用透射电子显微镜、紫外-可见-近红外分光光度计、荧光分光光度计、傅里叶红外光谱仪等表征手段对纳米复合材料的形貌、光学性质、化学组成、细胞毒性及荧光成像进行了研究.结果表明,水相AgInS2量子点的尺寸约为5nm~6nm,经叶酸(FA)修饰后,细胞毒性较低,在乳腺癌细胞中成像时红色荧光更明显,说明通过叶酸的修饰可提高量子点的生物兼容性.因此这类具有较好水溶性和生物兼容性的FA-AgInS2量子点可直接应用于生物成像研究,为乳腺癌的快速体外检测打下了良好的实验基础.%In order to synthesized ternary quantum dots with good water solubility,low toxicity and strong biocompati-bility,AgInS2 quantum dots were synthesized by aqueous phase synthesis and modified with folic acid(FA). Transmis-sion Electron Microscope,UV-Vis-NIR Spectrophotometer,Fluorescence Spectrophotometer,Infrared Spectrometer, Fourier transform infrared spectroscopy and other characterization methods were used to study the morphology,optical properties,chemical composition,cytotoxicity and fluorescence imaging of nanocomposites. The results showed that the size of the AgInS2 quantum dots in water was about 5~6nm,and the cytotoxicity was lower after folic acid(FA) modification. The red fluorescence in the breast cancer cells was more obvious,indicating that the biocompatibility of quantum dots can be improved by folic acid modification. Therefore,these FA- AgInS2 quantum dots with good water solubility and biocompatibility can be directly applied to the research of biological imaging,which lays a good founda-tion for rapid and in vitro detection of breast cancer.