补肺益肾方对幼龄哮喘豚鼠模型IL-5信号通路的调控机制

摘要

Objective To study the effect of the traditional Chinese medicine of invigorating lung and nourishing kidney on interleukin-5 level and expression of IL-5 receptorαmRNA in eosinophils( EOS)of bronchoalveolar lavage fluid( BALF)of young guinea pigs with asthma and to explore the mechanism of the traditional Chinese medicine of invigorating lung and nourishing kidney in promoting eosinophil apoptosis by IL-5 signal pathway. Methods The 24 young guinea pigs were divided into three groups randomly,normal control group,model group and the traditional Chi-nese medicine group. Asthma model of guinea pigs were sensitized by ovalbumin. EOS was purified from BALF. The content of IL-5 in BALF was detected by enzyme-linked immunosorbent assay( ELISA ). Apoptosis was detected by TdT-mediated dUTP nick end labeling(TUNEL). Expressions of IL-5 IL-5Rα mRNA were measured by bridization. Results The content of IL-5 in model group was higher than that of normal group( P﹤0 . 01 ),while the content of IL-5 in the traditional Chinese medicine group was lower than that of model group( P﹤0 . 05 ). EOS apoptosis in model group guinea pig were significantly decreased,while EOS apoptosis in the traditional Chinese medicine group guinea pig were higher than that of model group( P﹤0 . 01 ). Expressions of IL-5 RαmRNA in EOS from model group were re-markably lower than that of normal group( P﹤0 . 01 ),while expressions of IL-5 RαmRNA in EOS from the tradition-al Chinese medicine group were increased( P﹤0 . 05 ). Conclusion The traditional Chinese medicine of invigorating lung and nourishing kidney promotes eosinophil apoptosis by decreasing the content of IL-5 in airway and increasing expressions of IL-5 R α mRNA. So it can relieve airway inflammation and prevent asthma.%目的：观察中药补肺益肾方对幼龄哮喘豚鼠支气管肺泡灌洗液（BALF）中白介素-5（IL-5）水平以及嗜酸粒细胞（ EOS）上白介素-5受体α（ IL-5 R α）mRNA表达的影响，探讨中药补肺益肾方通过IL-5信号通路促进哮喘EOS凋亡的机制。方法24只健康幼龄豚鼠随机分为正常组、模型组、中药组。以卵蛋白致敏激发制备豚鼠哮喘模型，分离豚鼠BALF中的EOS，酶联免疫吸附法（ ELISA）检测BALF中IL-5的含量，TdT介导的dUTP缺口末端标记法（ TUNEL）检测EOS凋亡，原位杂交检测IL-5 RαmRNA表达。结果模型组IL-5水平高于正常组（ P﹤0.01），中药组IL-5水平低于模型组（ P﹤0.05）。模型组豚鼠EOS凋亡明显减少，中药组EOS凋亡明显高于模型组（ P﹤0.01）。模型组豚鼠EOS表达IL-5 Rα mRNA明显低于正常组（ P﹤0.01），中药组EOS表达 IL-5 RαmRNA明显增加（ P﹤0.05）。结论中药补肺益肾方可通过降低气道IL-5水平，促进IL-5 RαmRNA表达，来促进EOS凋亡，减轻气道炎症，从而达到防治哮喘的目的。