Notch3的表达与高血压肾纤维化

摘要

目的：检测Notch3在原发性高血压病人和自发性高血压大鼠肾中的表达，探讨Notch3与高血压肾纤维化的关系。方法：13例原发性高血压病人为高血压病人组(HP组)，15例肾肿瘤病人为对照组(CP组)。6只自发性高血压大鼠(spontaneous hypertensive rat，SHR)为高血压组(SHR组)，6只同龄WKY大鼠为对照组(WKY组)。Masson三色染色法检测SHR组和WKY组肾胶原纤维的表达；免疫组织化学染色法检测Notch3在病人和大鼠肾组织中的表达；实时PCR检测锌指转录因子(snail) mRNA在SHR组和WKY组肾中的表达。结果：SHR组肾间质胶原纤维较WKY组明显增多(P<0.05)；HP组Notch3的表达高于CP组(分别为6.741±0.231和0.763±0.358，P<0.001)，SHR组Notch3的表达高于WKY组(分别为5.487±0.774和0.421±0.163，P<0.001)；SHR组肾中snail mRNA的表达多于WKY组，差异有统计学意义(分别为0.996±0.120和0.208±0.090，P<0.01)。结论：Notch3可能与高血压肾纤维化的发生有关。%Objective:To examine the expression of notch3 in the kidneys of patients with primary hypertension and rats with spontaneous hypertension, and to explore the relationship of notch3 and hypertension renal ifbrosis.Methods:hTirteen patients with primary hypertension served as a primary hypertension group (HP group), and 15 patients with kidney tumor served as a control group (CP group). The spontaneous hypertensive rats served as a primary hypertension group (SHR group, n=6), and WKY rats served as a control group (WKY group, n=6). Masson stainning was used to examine the collagen in the kidneys in the SHR group and the WKY group. Immunohistochemical staining was used to detect the levels of Notch3 in kidneys of the patients and the rats. hTe expression of snail mRNA in the kidneys in the SHR group and the WKY group was examined by real-time PCR. n Results:Masson staining showed much more collagen in the SHR group than that in the WKY group (P<0.05);the expression of Notch3 in the HP group was much higher than that in the CP group ( 6.741±0.231 vs 0.763±0.358, P<0.01). The expression of Notch3 in the SHR group was much higher than that in the WKY group (5.487±0.774 vs 0.421±0.163, P<0.01), and hTe expression of snail mRNA was much higher in the SHR group than that in the WKY group (0.996±0.120 vs 0.208±0.090, P<0.01 ). n Conclusion:Notch3 may be related to the occurrence of hypertension renal ifbrosis.