研究了硬毛粗毛盖孔菌Funaliatrogii以稻草为培养基质进行固体发酵产生高活性漆酶。在筛选合适的碳源、氮源和硫酸铜浓度的基础上，开展四因素正交实验，筛选出最优培养方案，即以稻草粉为基质，淀粉0．5％、尿素0．3％、硫酸铜1％（质量分数），湿度为70％的条件下培养7天后漆酶活性即可达到145．42U／gds。在最优方案条件下连续培养，发现该菌在稻草粉培养基质上培养28天产生的漆酶活性能达到最高，为1518．76U／gds。而在增加培养规模的情况下，以最优方案培养28天的漆酶活性平均能达到1753．49U／gds。%Laccase production ofFunalia trogii by rice-straw solid-state fermentation was studied. On the base of choosing optional carbon sources, nitrogen sources and optional content of CuSO4, four factors design experiments were carried out with 2 g rice stalk substrate. A best design has been chosen out which including 2 g rice-stalk powder, content of 0.5% starch, 0.3% urea, 1% CuSO4 and 70% moisture and the laccase activity is up to 145.42 U/gds after 7 d. However, the highest laccase activity could be achieved at 28 d with the best design, which is 1 518.76 U/gds. For the aim of practiced application, 10 g rice stalk powder experiment were carried out and the good results were repeated.
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