大鼠肝脏组织视黄醇结合蛋白4在衰老过程中表达及与胰岛素抵抗的关系

摘要

Objective;To investigate difference expression of retinol binding protein 4( RBP4) during aging in liver tissue of rat and explore the molecular mechanism of insulin resistance(IR). Methods: We used the minimal model technique of Bergman to estimate the insulin sensitivity of young(8 - 12 weeks) and aged (24months) rat. The levels of RBP4 and peroxisome proliferator-activated receptora ( PPARα) mRNA were measured by reverse transcription-polymerse chain reaction ( RT-PCR) and the proteins of RBP4 and PPARα were determined by western blotting,respectively. Results;The degree of IR in the aged group was significantly increased compared with that in the young group. The RBP4mRNA and protein of the aged group were significantly increased. The PPARα mRNA and protein of the aged group were significantly decreased. Conclusion: The mechanism of IR during aging is probably associated with the increased expression of RBP4 in rat liver.%目的:观察大鼠肝脏组织视黄醇结合蛋白4( retinol binding protein4,RBP4)在衰老过程中表达差异,在分子水平上探讨衰老过程中出现胰岛素抵抗(insulin resistance,IR)的可能机制.方法:用Bergman创立的微小模型技术评价年轻鼠(8～12周龄)和老年鼠(24个月龄)的胰岛素敏感性,取大鼠肝脏组织提取总RNA,采用半定量RT-PCR法检测RBP4和过氧化体增殖物激活型受体α(peroxisome proliferator-activated receptorα,PPARα)mRNA水平,用Western blot法检测RBP4和PPARα蛋白表达情况.结果:与年轻鼠组比较,老年鼠组存在明显的胰岛素抵抗,而老年鼠组的RBP4mRNA及蛋白表达明显升高,PPARαmRNA及蛋白表达明显减少.结论:衰老过程中大鼠肝脏组织RBP4表达升高可能与胰岛素抵抗有关.