光动力治疗中氧分压和微弱荧光监测及成像系统的研究

摘要

Since the key multiple parameters need to be monitored during the photodynamic therapy (PDT)in deep tumor,we de-veloped a pO2 and weak fluorescence monitoring and imaging system.The Princeton Instrument scientific -grade CCD was used as the fluorescence imaging detector,NeoFox of Ocean Optics as O2 sensor and LabVIEW as programming platform.The function of the sys-tem included the fluorescence image measurement,the pO2 data acquisition,the fluorescence intensity detection,result display,data analyzing and recording simultaneously.The system realized the real -time multiple parameters measurement and analysis during PDT. The PDT chemical reaction experiments validate the sensitivity of system,which has a potential for assessment of PDT.%针对深部肿瘤光动力治疗（PDT）过程中关键参数的采集，我们开发了一套实时在体的氧分压和微弱荧光监测及成像系统。该系统利用普林斯顿高灵敏度 CCD 采集微弱荧光图像，利用海洋光学氧传感器NeoFox 监测氧含量，采用 LabVIEW 开发上位机控制程序，将荧光图像、氧分压、荧光强度数据、结果的显示、分析与存储等功能结合在一起，实现了光动力治疗过程中实时多参数监测与成像。经光动力治疗反应荧光实验，验证了系统的有效性，为后续 PDT 疗效评估研究工作奠定了基础。