太子参超低温脱毒及规模化组培育苗技术

摘要

The shoot tips of Pseudostellaria heterophylla as explants in vitro culture was conducted for adventitious bud induction , bud multiplication and root induction under super low temperature treatment, in order to form a large-scale breeding technology .The results showed that with super low temperature treatment for 1 hours, radix Pseudostellariae virus-free rate might reach above 90%.The most suitable initial culture medium in the fast reproduction system was MS +2.5 mg/L 6-BA+0.5 mg/L IAA, and the subculture medi-um was MS+1.2 mg/L KT+0.5 mg/L NAA.The highest proliferation rate was 12.5.The rooting medium was MS +0.2 mg/L KT+1.5 mg/L DA-6+10%protein powder .%以太子参茎尖为外植体，采用超低温去除病毒方法，通过组织培养研究了芽增殖诱导、丛生芽诱导、生根壮苗诱导的适合条件，以期形成太子参规模化育苗技术。结果表明：超低温处理1 h后，太子参脱毒率可达90％以上；规模化组培育苗最适宜配方为初代培养基为改良MS＋2.5 mg/L 6-BA＋0.5 mg/L IAA继代增殖培养基为改良MS＋1.2 mg/L KT＋0.5 mg/L NAA，壮苗生根培养基为改良MS＋0.2 mg/L KT＋1.5 mg/L DA-6＋10％蛋白粉。