在淡水和海水环境下虹鳟血液理化指标、肌肉和肝脏的抗氧化酶活性的比较

摘要

Using Beckman electrolyte and automatic biochemical analyzer , we compared the blood index , and the activity of antioxi-dant enzyme in muscle and liver under different circumstance .It turned out that under sea and fresh water environment , the content of glutamic pyruvic transaminase(GPT)are significantly different(P<0.05), so are the contents of aspartate transaminase (GOT).The contents of fish in GDT and GOT in freshwater are higher than those of seawater , the values are ( 7.00 ±2.82 )μ/L and ( 44.00 ± 7.43)μ/L, respectively, which are 1.36 times and 3.35 times of the seawater group.The trace element potassium (K+), sodium (Na+), chlorine(Cl-), calcium (Ca2+) and albumin(A), triglycerides and globulin(G)in sea wafer fish are greater than those in freshwater group .Total cholesterol levels were markedly different ( P <0.05 ) , the level of seawater group is greater than that of fresh-water group.Hemoglobin, platelet hematocrit, number of red blood cells, platelet count of fishes in seawater are obviously higher than those in freshwater group , which decreased by 68.31%, 37.32%, 22.58%and 24.29%, respectively;The width of platelet distribu-tion and red blood cell distribution and the hematocrit is apparently different ( P<0.05 ) .%采用贝克曼电解质分析仪和日立H－7600全自动生化分析仪，对不同环境下虹鳟血液理化指标、肌肉和肝脏抗氧化酶活性进行比较。结果表明：1）海、淡水环境下，虹鳟血清中谷丙转氨酶（ GPT ）含量差异显著（ P＜0．05），谷草转氨酶（GOT）含量差异也显著（P＜0．05），淡水组均大于海水组，其值是分别是（7．00±2．82）μ／L、（44．00±7．43）μ／L，分别是海水组的1．36倍和3．35倍。2）微量元素钾（K＋）、钠（Na＋）、氯（Cl －）、钙（Ca2＋）和白蛋白（ A）、甘油三酯以及球蛋白（ G）含量，海水组大于淡水组；总胆固醇含量差异显著（ P＜0．05），海水组大于淡水组。3）海水组血红蛋白含量、血小板压积、红细胞数目、血小板数目均大于淡水组，其值分别降低了68．31％、37．32％、22．58％、24．29％；血小板分布宽度、红细胞分布宽度、红细胞压积差异显著（ P＜0．05）。