复方护肝汤对刀豆蛋白A诱导小鼠肝损伤的保护作用

摘要

目的:探讨复方护肝汤对刀豆蛋白A(ConA)诱导的小鼠急性肝损伤的保护作用及其可能作用机制.方法:将BALb/c小鼠随机分为正常对照组、ConA诱导肝损伤组、联苯双酯治疗组(1.5 mg·kg-1·d-1)及复方护肝汤低、中、高剂量(7、14、28 g·kg-1·d-1)组,每组10只.尾静脉注射ConA 12.5 mg/kg,每周1次;对照组尾静脉注射250 μl PBS溶液,每周1次.均连用7周.除对照组外,其余各组均诱导小鼠急性肝损伤模型.分别检测各组小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、谷氨酰转肽酶(GGT)、透明质酸酶(HA)和肝组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)的水平.结果:经复方护肝汤治疗后,ConA诱导肝损伤小鼠的血清ALT、AST、ALP、GGT、HA和肝组织匀浆MDA、NO的水平均比模型组减低(P<0.05～P<0.01);中、高剂量组血清SOD水平均较模型组显著增高(P<0.01).结论:复方护肝汤对ConA诱导小鼠急性肝损伤具有较好的防治功能,其作用机制可能与其抗氧化活性有关.%Objective: To investigate the effects of compound Hugan decoction on conA-induced liver injury in mice. Methods: BALb/c mice were randomly divided into six groups, 10 in each, which included normal, liver injury ,biphenyldimethylesterate treatment 1.5 mg· kg-1 · d-1 and compound Hugan decoction treatment(7 g · kg-1 · d-1, 14 g · kg-1 · d-1 ,28 g · kg-1 · d-1) groups. The animal model of liver injury was established by intravenous injection of ConA ( 12. 5 mg/kg) in BALb/c mice. The serum level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamyltransferase (GGT), and hyaluronidase(HA) were assayed by spectrophotometer. Results:Compound Hugan decoction significantly decreased the concentration of serum transaminases(ALT,AST,ALP,GGT) and it showed a dose-dependent manner between the range of 7 -28 g · kg-1 · d-1.Conclusions:Compound Hugan decoction performs protective effect against ConA-induced liver injury in mice. Such protective effects might be related to the increase of antioxidant enzyme activity and the promotion of free radicals elimination.