吉林、兰州地区婴幼儿人星状病毒腹泻的分子流行病学研究

摘要

目的:研究吉林、兰州地区婴幼儿人星状病毒(HAstV)腹泻流行情况和分子特征.方法:对吉林地区417份和兰州地区290份腹泻标本采用one-step PCR方法检测HAstV,用HAstV基因组ORF1b区部分核苷酸片段初筛,再用ORF2区部分核苷酸序列分型.结果:707份腹泻标本中共检出HAstV阳性17例,总阳性率为2.4%,其中吉林地区和兰州地区分别检出7例和10例.12份HAstV ORF2区阳性的样本均为HAstV 1型,除1株属于Lineage 1a群,其他均属于Lineage 1b群.HAstV主要感染2岁以下婴幼儿,在吉林春季为主要流行季节,而在兰州春季和秋季为主要流行季节.结论:HAstV是引起婴幼儿病毒性腹泻的主要病原之一,HAstV 1型是主要的流行株,2岁以下为感染发病的高危人群,不同地区HAstV流行季节不同.%Objective: To explore the prevalence status and molecular features of astrovirus diarrhea in infants from Jilin and Lanzhou. Methods: The astrovirus expressions in 417 diarrhea specimens from Jinlin and 290 specimens from Lanzhou were detected by one-step PCR. Partial nucleotide fragments in ORF1 b region of astrovirus genome were screened, which were somatotypedbg using partial ORF2 sequences. Results: Seventeen postive astrovirus cases in 707 diarrhea specimens, which included 7 cases from Jilin and 10 cases from Lanzhou were detected,the total postive rate was 2. 4%. The postive ORF2 specimens in 12 cases were HAstV 1 , which belonged to Lineage 1 b cluster except 1 Lineage 1 a cluster. The HAstV infection mainly distributed in infants less than 2 years, the main morbidity season in Jinlin and Lanzhou were Spring and Autumn, respectively. Conclusions: Astrovirus is the main pathogen of virus diarrhea in infants, HAstV 1 is common serotype, children less than two years old are high-risk group and main morbidity season is difference in different areas.