三氧化二砷对HL-60细胞端粒酶hTERT启动子活性的影响

摘要

目的:探讨三氧化二砷(As2O3)抑制HL-60细胞端粒酶hTERT表达的分子机制.方法:构建含有hTERT启动子不同片段的荧光素酶基因表达载体,转染HL-60细胞,分别在有无As2O3作用下检测各组荧光素酶活性变化,间接反映As2O3对hTERT启动子不同片段活性的影响.结果:在As2O3作用下,含有hTERT启动子不同片段的荧光素酶基因表达载体的荧光素酶活性均下降(P<0.05～P<0.01).结论:As2O3对HL-60细胞端粒酶hTERT启动子活性具有抑制作用.%Objective: To investigate the molecular mechanism of arsenic trioxide (As2O3) inhibiting telomerase human telomerase reverse transcriptase ( hTERT) expression in HL-60 cells. Methods: The different fragments of hTERT promoter luciferase gene expression vector were constructed and transfected into HL-60 cells with or without As2O3. The changes of luciferase activity which indirectly reflected the role of As2O3 on hTERT promoter different fragments were detected. Results:The luciferase activities in different fragments of hTERT promoter gene expression vector were decreased with As2O3 (P<0. 05 to P<0. 01). Conclusions: As2O3 can inhibit telomerase activity of hTERT promoter in HL-60 cells.