辛通畅络疏利祛浊法治疗大鼠局灶性节段性肾小球硬化的研究

摘要

目的：观察以辛通畅络、疏利祛浊法中药对大鼠局灶性节段性肾小球硬化的治疗作用及作用机制。方法采用2次阿奇霉素尾静脉注射方法复制局灶性节段性肾小球硬化模型，按体重均衡后随机分为正常组、模型组、治疗组（西药组、中药组），每组15只。各组动物于造模第9周末开始给药，西药组予贝那普利，中药组予辛通畅络疏利浊方药，连续8周。于实验的第1、4、8周末测大鼠24 h 尿蛋白，血清白蛋白、肌酐、尿素氮含量，并进行光镜、电镜等观察。结果治疗组各项指标较模型组均有显著性差异，病理学观察也显示治疗组肾小球损伤均轻于模型组。结论中药组能够明显保护大鼠的肾功能，明显减轻模型组大鼠细胞外基质沉积，减轻肾小球硬化，延缓慢性肾脏病的进展。%Objective To study the therapeutic effect and mechanism of Xintong Changluo Shuli Quzhuo therapy (hereinafter called “TCM”)against rats with focal segmental glomerular sclerosis in rats. Methods The rat model of focal segmental glomerular sclerosis was induced by tail intravenous injection of azithromycin twice.According to their weight,all rats were divided into normal group,model group, benazepril group and TCMgroup (n =15 each).At the end of 9 th week after modeling,the correspond-ing drugs were administered orally.Then at the 4th week and at the 8 th week after treatment,24 h urine protein,serum albumin,creatinine and urea nitrogen levels were measured and morphological changes were observed under light microscope and electron microscope.Results Compared with model group, each index of TCM group had significant differences,and pathological observation showed that the glo-merular injury in TCMgroup was slighter than that in the model group.Conclusion Xintong Changluo Shuli Quzhuo therapy can obviously protect the renal function of rats,significantly reduce extracellular matrix deposition and glomerular sclerosis,delay the progress of chronic kidney disease.