目的：建立高山大黄药材的质量控制方法。方法采用薄层色谱法，以白皮杉醇-4′-O-β-D-葡萄糖苷为对照品对高山大黄药材进行薄层鉴别。按照2010版《中国药典》附录IX方法对高山大黄药材进行水分、总灰分及酸不溶灰分的测定。采用HPLC法同时测定高山大黄中白皮杉醇-4′-O-β-D-葡萄糖苷和白藜芦醇-4′-O-β-D-葡萄糖苷的含量。结果10批高山大黄中白皮杉醇-4′-O-β-D-葡萄糖苷在所建立的色谱条件下分离效果好，斑点清晰。10批高山大黄药材的水分含量在7．46%~9.14%，总灰分含量在2.72%~8.75%，酸不溶灰分含量在0.50%~2.51%。10批高山大黄药材中白皮杉醇-4′-O-β-D-葡萄糖苷的含量在1.768~70.720 mg/L范围内呈良好的线性关系( r=0.9998)，加样回收率为101.11%( RSD=0．89%)；白藜芦醇-4′-O-β-D-葡萄糖苷的含量在0．416~16.640 mg/L 的范围内呈良好的线性关系( r=0.9996)，加样回收率为99.68%( RSD=2．54%)。结论高山大黄药材的薄层鉴别方法和HPLC含量测定方法简便、准确、重复性好，可作为高山大黄药材的质量控制方法。%Objective To explore the method to control the quality of Rheum nobile. , a widely used Tibet-an herbal medicine. Methods The herb powder of ten batches of Rheum nobile was identified by thin layer chromatography ( TLC) with piceatannol-4′-O-β-D-glucopyranoside as the reference substance, and the moisture content, total ash and acid-insoluble ash were detected in accordance with Appendix IX of Chinese Pharmacopoeia 2010 Edition. The contents of piceatannol-4′-O-β-D-glucopyranoside and res-veratrol-4′-O-β-D-glucopyranoside were determined by using HPLC. Results The spots of piceatannol-4′-O-β-D-glucopyranoside of ten batches of Rheum nobile were clear and well separated. The range of moisture content was from 7 . 46% to 9 . 14%, and that of total ash was 2 . 72% to 8 . 75%, and that of acid-insoluble ash 0 . 50% to 2 . 51%. The content of piceatannol-4′-O-β-D-glucopyranoside was showed a good linear relationship at the range of 1 . 768 to 70 . 720 mg/L ( r=0 . 999 8 ) and the recovery rate was 101. 11% ( RSD =0. 89%), by parallel, that of resveratrol-4′-O-β-D-glucopyranoside was showed a good linear relationship at the range from 0 . 416 to 16 . 640 mg/L ( r=0 . 999 6 ) and the recovery rate was 99. 68% (RSD=2. 54%). Conclusion The methods of TLC and HPLC are simple, accurate and re-peatable, which could be the option of the quality control of Rheum nobile.
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