蜘蛛香成分11-ethoxyviburtinal对肠易激综合征大鼠肠嗜铬细胞及肥大细胞表达的影响∗

摘要

Objective To investigate the effects of 11-ethoxyviburtinal of Valeriana jatamansi Jones on the expression of enterochromaffin cells ( EC) and mast cells ( MC) in rats with irritable bowel syndrome ( IBS) . Methods The IBS rat model was induced by chronic unpredictable stress with isolation. Male SD rats were randomly divided into six groups:control group,model group, pinaverium group at the dose of 25 mg/kg,and high-, mid-and low-dose of 11-ethoxyviburtinal group at dose of 1. 2, 0. 6, and 0. 3 mg/kg respectively. The number of 5-HT positive immunoreactive colonic EC was detected by the method of immunohistochemistry and that of colonic MC was measured with toluidine blue staining. Results Compared with control group, the number of 5-HT positive immunoreactive colonic EC and MC increased significantly in model group ( P <0 . 05 ) . The high dose of 11-ethoxyviburtinal could significantly de-crease the number of colonic EC and MC ( P<0 . 05 ) . Conclusion The 11-ethoxyviburtinal of Valeri-ana jatamansi Jones could significantly reduce the number of 5-HT positive immunoreactive 5-HT positive immunoreactive EC and MC in colon tissue of rats with IBS.%目的：探讨蜘蛛香成分11-ethoxyviburtinal对肠易激综合征( IBS)大鼠肠嗜铬细胞( EC)及肥大细胞(MC)表达的影响。方法 SD 雄性大鼠随机分为空白对照组、模型组、匹维溴铵(25 mg/kg)组和蜘蛛香成分11-ethoxyviburtinal大、中、小剂量(1.2、0.6、0.3 mg/kg)组。采用孤养复合慢性不可预见性应激法建立IBS 大鼠模型，免疫组化法检测大鼠结肠5-羟色胺(5-HT)免疫反应阳性细胞EC数量的变化，甲苯胺蓝染色法检测大鼠结肠MC数量的变化。结果孤养复合慢性不可预见性应激可造成大鼠结肠5-HT 免疫反应阳性细胞EC数量显著增加( P<0.01)，结肠MC数量显著增多( P<0．05)。蜘蛛香成分11-ethoxyviburtinal大剂量可显著减少结肠5-HT免疫反应阳性细胞EC和MC数量( P<0.05)。结论蜘蛛香成分11-ethoxyviburtinal可抑制IBS大鼠结肠EC和MC的表达。