猪细环病毒2型感染与断奶后多系统衰竭综合征的相关性分析

摘要

[Objective]To analyze the association between Torque teno sus virus type 2 (TTSuV2) infection and postweaning multisystemic wasting syndrome (PMWS).[Method]The sera samples of 35 PMWS-suspected pigs and 35 age-matched healthy pigs from the same group were collected,and the comprehensive diagnosis was used to confirm the PMWS-suffered pigs and sub-clinical porcine circovirus type 2 (PCV2)-infected pigs.The virus load of both PCV2 and TTSuV2 was determined with fluorescent quantitative PCR,and the difference in virus load of the two viruses was compared,respectively.Moreover,the load correlation between the two viruses was analyzed.[Result]The comprehensive diagnosis confirmed that there were 24 PMWS-suffered pigs and 27 sub-clinical PCV2-infected pigs.The virus load of both PCV2 and TTSuV2 in PMWS-suffered pigs were all significantly higher (P＜0.01) than sub-clinical PCV2-infected pigs,and there was a significant (P＜0.01) correlation between TTSuV2 load and PCV2 load.[Conclusion]There was a certain clinical correlation between PMWS and TTSuV2 infection.%[目的]分析猪细环病毒2型(TTSuV2)感染与猪断奶后多系统衰竭综合征(PMWS)的发生是否存在临床相关性.[方法]采集35头临床疑似PMWS病猪和35头同群健康猪的血清,用综合诊断方法来确认PMWS病猪与猪圆环病毒2型(PCV2)亚临床感染猪,用荧光定量PCR方法检测PCV2与TTSuV2载量,对2种病毒载量分别进行差异分析,并对二者进行相关性分析.[结果]经综合诊断确认,有24头PMWS病猪和27头PCV2亚临床感染猪,前者血清中PCV2载量与TTSuV2载量都极显著(P＜0.01)高于后者,且TTSuV2载量与PCV2载量之间存在极显著(P＜0.01)相关.[结论]PMWS与TTSuV2感染在临床上存在一定相关.