野山参与园参rDNA-ITS序列比较分析

摘要

Objective The difference of rDNA-ITS sequences between wild ginseng and cultivated ginseng was compared for building DNA molecular identification method.MethodThe total DNA of wild ginseng and cultivated ginseng was extracted.ITS sequences of both were amplified and analyzed by ClustalX software.ResultsThe PCR results of wild ginseng and cultivated ginseng obtained two bands,700 bp and 500 bp respectively.There was no difference between wild ginseng and cultivated ginseng in the sequencing of 700 bp band and only 38% similarity in the sequencing of 500 bp band.ConclusionThis results provided the experimental bases for the identification.%目的 比较野山参与园参rDNA-ITS序列差异,寻找其DNA分子鉴定方法.方法 提取野山参与园参总DNA,扩增其ITS序列,运用ClustalX等软件比较分析野山参与园参ITS序列特征.结果 野山参与园参经PCR扩增后分别获得700 bp 和500 bp 两条条带.其中对野山参和园参的700 bp 条带测序分析后未发现差异位点,而二者的500 bp 条带测序结果相似性仅为38%. 结论 该结果为野山参的鉴别提供了实验依据.