庆大霉素对豚鼠血管纹Na，K-ATP酶α亚单位异构体表达的影响

摘要

目的观察正常豚鼠血管纹Na，K-ATP酶α亚单位异构体的表达及庆大霉素对其表达的影响。方法用鼠抗大鼠Na，K-ATP酶α亚单位异构体特异性单克隆抗体，采用免疫组化SP法观察正常豚鼠血管纹中Na，K-ATP酶α1异构体的表达及庆大霉素作用后表达的变化。结果正常豚鼠血管纹Na，K-ATP酶α1异构体表达呈阳性，而α2和α3异构体表达呈阴性。庆大霉素作用后α1异构体表达显著减弱。结论庆大霉素显著抑制豚鼠血管纹Na，K-ATP酶α异构体的表达，从而抑制血管纹Na，K-ATP酶的活性。%Objective To investigate the expression of asubunit isforms of Na,K-ATPase in stria vascularis of guinea pig, and the effect of gentamicin on it. Methods The expression of a subunit isoforms of Na, K-ATPase in stria vascualris of guinea pig and its change after gentamicin exposure were identified immunocytochemically using the specific mouse antibodies a gainst the α1 ,α2 and α3 subunits of rat Na,K-ATPase. Results All marginal cells,intermediate cells and basal cells of stria vas cualris showed positive expression of α1 isoform and negative expression of α2 and α3 isoforms. The immunostaining of α1 isform was attenuated significantly after gentamicin esposture. Conclusion Gentamicin significantly inhibits the expression of Na, K-ATPase α1 subunit isoform of the stria vascularis, sequentially inhibits the activity of Na,K-ATPase.