[目的] 为农村生活污水的净化处理提供菌源和技术支持.[方法] 从生物净化器/强化浮床组合工艺处理农村生活污水工程的三格厌氧氧化槽中提取菌种,并在筛选培养基中复筛出3株高效活性菌株.经过生理生化试验和优化条件选择试验筛选出优化菌株.[结果] 将3槽内菌株富集后各自初步筛选出3株菌株A-1、A-2、A-3,B-4、B-5、B-6,C-7、C-8、C-9.在一定培养条件下根据总氮、总磷和COD的变化筛选出高效菌株A-1、B-5、C-7作为优化试验的菌种.A-1、B-5、C-7的1∶1∶1混合培养菌株D-X因单株之间的协同作用使其脱氮除磷速率比单株都高, 故选用D-X作为生物脱氮除磷的接种剂.经优化,D-X的最佳培养条件:在300 ℃和厌氧条件下,pH值为7.0左右,N/P为6,接种量为20%.[结论] 优选出了混合培养菌株D-X,该菌株脱氮除磷效果明显,可广泛用于农村生活污水的处理.%[Objective] The technical support and the bacteria sources for the purification of life sewage in rural were provided. [Method] The bacteria source extracted from triple-vessel anaerobic oxidation tank for the treatment of the domestic sewage in rural area was purified with the technical combination of biological purification instillation and floating-bed. 3 strains with high activity were isolated from the media. The optimal strain was selected through the physiological and biochemical test and optimal test condition. [Results] 3 strains of A-1, A-2, A-3, B-4, B-5, B-6, C-7, C-8 and C-9 were preliminarily screened out from 3 tanks where the strains were accumulated, respectively. The high-performance strains: A-1, B-5 and C-7 were selected according to total nitrogen, total phosphorus and COD under certain culture condition. The D--X of the mixed culturing strain of A-1, B-5, C-7(1∶1∶1) had the higher rate of nitrogen and phosphorus removal than these of single strain because of their synergy. Thus, the D-X of the mixed strain could be selected as the inoculants of biological nitrogen/phosphorus removal. After optimized, the best culturing condition of D-X was: the temperature, 300 ℃; the environment, anaerobic culture; the pH value, about 7.0; the rate of N/P, 6 and the inoculation amount, 20%. [Conclusion] The D-X of mixed strain was best with obvious effect on the nitrogen and phosphorus removal, which could be widely used in the treatment of domestic sewage in rural area.
展开▼
