药用植物仙茅的组织培养及快繁体系的建立

摘要

[Objective]In order to preserve the natural resources of Curculigo orchiodes Gaertn which has been destroyed seriously,the method of tissue culture has been systematically studied. [Method]The explants of immature leaves were cultured on MS culture media by adding different portions of hormones at various cultural conditions. [Result]The suitable phytohormone combination to induce callus were MS+6-BA 0.50 mg/L+NAA 0.05 mg/L or MS+6-BA 1.00 mg/L+NAA 0.05 mg/L;For the adventitious buds and roots, they were MS. [Conclusion]Tissue culture of C.orchiodes make its propagation rapid, its resources preserved,and its utilization last.%[目的]针对仙茅市场需求量不断增长,野生资源受到严重破坏的情况,系统地研究了药用植物仙茅的组织培养及快繁体系的建立.[方法]以仙茅根茎上的幼叶为外植体,在MS培养基上添加不同的激素配比进行组织培养.[结果]外植体在MS+6-BA 0.50 mg/L+NAA 0.05 mg/L或MS+6-BA 1.00 mg/L+NAA 0.05 mg/L培养基中培养20 d诱导产生愈伤组织;将诱导产生的愈伤组织转入不含激素的MS培养基中培养30 d诱导产生芽,同时在芽的基部产生根;幼苗的移栽成活率高达95%以上.[结论]采用组织培养方式可进行仙茅的快速繁殖,为确保药用植物资源仙茅的保护和可持续利用提供有效途径.