首页> 中文期刊> 《安徽农业科学》 >油菜秸秆降解菌群的筛选及降解特性研究

油菜秸秆降解菌群的筛选及降解特性研究

         

摘要

[目的]筛选降解油菜秸秆的最优菌群,并研究其降解特性.[方法]通过刚果红平板法、苯胺兰平板法及纤维素酶、木质素酶产酶试验考察了6个菌群的油菜秸秆降解能力.[结果]腐烂秸秆土菌群生长最快,具有最大的生长量,木质素酶活性最高,纤维素酶活性达到了924 U/ml.腐烂秸秆土菌群固态发酵降解油莱秸秆14d,对油莱秸秆、纤维素、木质素降解率为71%、82%、53%.前12d对油莱秸秆、纤维素、木质素的降解量分别占各自总降解量的86.05%、93.35%、93.15%.[结论]腐烂秸秆土菌群为降解油莱秸秆最优菌群,并且在降解的前12 d为高效降解阶段.%[Objective]The study was to select the dominant degrading microbial community of rape straw,and analyzed their characteristics. [Method] According to congo red and aniline blue plate,cellulose and ligninase producing test,straw -degrading microbial community with the best degradation efficiency were choosen from 6 natural flora. [ Result]The microbial community from rotten rape straw had the highest ligninase activity,the activity of cellulase reached 924 U/ml. After 14 d of solid - state fermentation with rape straw by microbial community from rotten rape straw,the degradation rate of rape straw .cellulose and lignose reached 71% ,82%and 53% ,respectively. During the first 12 d,86.05% of rape straw,93. 35% of cellulose and 93. 15% of lignose was degradated respectively. [ Conclusion j The microbial community from rotten rape straw was the dominant degrading microbial community, the first 12 d of degradation was the efficient stage.

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