分光光度法测定角果藜中总黄酮含量

摘要

[Objective] The aim was to establish the method for determining total flavoens content in Ceratocarpus arenarius L.. [ Method] Flavonoids were extracted from C. Arenarius by Heating refluxing, and determined with rutin as the standard. [ Result] In the range of 0.019 - 0.102 mg/ml,linear relationship was good (r =0.9991). This method had higher precision and accuracy with RSD of 0. 226% and recoveries of 99. 36%. The total flavoens content from C. Arenarius is 10. 12 mg/g. [Conclusion] The method was simple and reliable, which could be used to control the quality of C. Arenariu.%[目的]建立角果藜中总黄酮含量的测定方法.[方法]采用加热回流法提取角果藜中的黄酮类化合物,以芦丁为标准品测定总黄酮含量.[结果]采用紫外分光光度法测定总黄酮含量时,在0.019 ～0.102 mg/ml浓度范围内线性关系良好(r=0.999 1).方法的精密度和准确度均较高,相对标准偏差为0.226％,平均回收率为99.36％.测得角果藜中总黄酮含量为10.12 mg/g.[结论]该方法操作简单,科学准确,适合用于角果藜中总黄酮含量的测定.