高CO2浓度对春兰叶片叶绿素·叶绿素蛋白质复合物含量的影响

摘要

[目的]为了研究春兰适应环境变化的方法.[方法]对自然状态(正常组,CO2浓度(370±50) μl/L)和高CO2浓度(试验组,CO2浓度(700±50) μl/L)春兰叶片的叶绿素及叶绿素蛋白质复合物含量进行研究.[结果]与正常组相比,试验组春兰叶片的叶绿素含量增高,叶绿素a/b降低；叶绿素蛋白质复合物的含量增加,经SDS-聚丙烯酰胺凝胶电泳得到6条叶绿素蛋白质复合物带和l条游离色素带,表明叶绿素蛋白质复合物种类未发生变化.从电泳结果可以看出,叶绿素蛋白质复合物聚合态如LH CⅡ3的量增多,单体态LHCⅡ1和LHCⅡ2的量减少.[结论]叶绿素、叶绿素蛋白质复合物含量的变化是春兰对高CO2浓度的一种适应效应,有利于提高其在光合作用中光能的吸收、传递和转换的效率,并且支持高效的光合碳素同化作用.%[Objective ] The research aimed 10 study the method of Cymbidwm goeringil adapting lo environmental change. [Method] The effects of gigg CO2 concentration on the cgloropgtkk and chlorophyll-protein complpexes af (Cymbidium goeringii leaves were studied. [ Result] Compared with the normal CO2 concentration {370 ±50 μ/L) ,the contents of chlorophyll and chlorophyll-proiein increased at high CO2 concentration (700 ±50 μ/L). The contents of polymer slate of PS Ⅱ light-harvesting chlorophyll a/b-protein complex (LHCⅡ } increased as the monomer state of LHC Ⅱ decreased. The changes of the contents of chlorophyll and chlorophyll-protein,and the state of LHC Ⅱ in Cymbutium goeringii were an adaptive effects of plant photosynthetic mechanism lo the long term elevated CO,. [Conclusion] These changes increased the efficiency of the absorption, transfer and conversion of light energy in plant photosynthesis, and support the high efficiency of pholosynthetic carbon assimilation.