[目的]探讨废次烟叶中茄尼醇的提取纯化工艺.[方法]采用超声波辅助提取技术,结合柱色谱法对废次烟草中茄尼醇的提取、分离和纯化进行了研究.[结果]通过正交试验获得茄尼醇最佳提取条件为:超声功率120 W,超声时间60 min,料液比1∶10,提取温度70℃.提取液经皂化、酸化后,用正己烷萃取得到纯度为21.5％的茄尼醇粗品;粗品经重结晶后得到纯度为77.6％的茄尼醇产品;进一步通过柱色谱分离,石油醚-乙酸乙酯(8/1,V/V)洗脱,收集相关流分,结晶得到纯度为99.2％的茄尼醇产品,整个工艺过程中茄尼醇产品的得率为0.512％.[结论]应用超声波辅助提取废次烟叶中的茄尼醇,具有萃取速度快、效率高的优点,适合工业化生产.%[Objective] The aim was to study isolation and purification technology of solanesol from discarded tobacco. [ Method] The extraction , isolation and purification technology of solanesol from discarded tobacco were studied by adopting ultrasonic-assisted extraction technique and column chromatography. [ Result] The optimum extraction conditions of solanesol from discarded tobacco were obtained through orthogonal experiments as follows; the ultrasonic power was 120 W; the extraction time was 60 min; the proportion of raw materials and solution was 1: 10; and the extraction temperature was 70℃. Then solansol was extracted from solansol extract by hexane with purity of 21.5% after saponification and acidification; after that the solansol with 77.6% purity was obtained by recrystallization; finally the crude solansol was purified on silica column chromatography using petroleun ether/ethyl acetate (8/1, V/V) as mobile phase. The purity of solanesol was 99.2% , and the yield of solanesol was 0.512%. [ Conclusion] Ultrasonic-assisted extracting solanesol from discarded tobacco has advantages of fast speed and high efficiency, so it is suitable for industrial production.
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