Using the urban river sediments, denitrifying phosphorus removal system was successfully acclimated by anaerobic/ anoxic/ aerobic ( A2O) process. Based on the system, denitrifying phosphorus accumulation bacteria were screened with BTB, metachromatic granules and PHB (poly-β-hydroxybutyrate ) staining experiments,and their genetic backgrounds were found out by 16S rDNA sequence analysis technology. Under the experimental conditions,denitrification phosphorus removal system had more than 80% removal efficiency of nitrogen and phosphorus,and three denitrifying phosphorus bacteria were isolated from the system,which were named DPB-A511 ,DPB-A9 and DPB-A10 separately and had more than 50% nitrogen and phosphorus removal efficiency. DPB-A511 and DPB-A9 had 97% similarity with Dechloromonas aroirudica ,Candida-tus accumulibacter plwsphatis, and DPB-A10 had 99% similanty with Bacillus pumilus.%利用城市河道底泥,通过厌氧/缺氧/好氧(A2O)工艺驯化反硝化除磷系统,采用BTB培养基、异染粒及PHB(聚β-羟基丁酸)染色等方法,从反硝化除磷系统中分离筛选出反硝化聚磷菌,并通过16S rDNA序列测定分析其遗传背景.在该试验条件下,反硝化除磷系统的氮磷的去除率超过80%.从反硝化除磷系统中分离筛选出DPB-A511、DPB-A9和DPB-A10 3株反硝化聚磷菌,其氮、磷去除率均超过50%.这3株菌中DPB-A511、DPB-A9分别与Dechloromonas aromatica、Candidatus accunuulibacter phosphates的相似性均达到97%,DPB-A10与Bacillus pumilus的相似性达到99%.
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