[目的]研究绿玉树茎段组织培养再生苗的条件,确定各培养阶段的最佳培养条件,为绿玉树组培苗工厂化生产和相关研究提供参考.[方法]以绿玉树茎段作为外植体试验材料,研究了不同培养基对萌芽率、增殖倍数、生根率的影响.[结果]萌芽培养最佳的诱导培养基为1/2MS+0.02mg/L NAA+1.00mg/L 6-BA,分化率为89.7%;继代培养最佳培养基为1/2MS+0.02mg/L NAA+0.60mg/L6-BA+3.00mg/L AD,增殖倍数为5.70;生根培养最佳培养基为1/2MS+0.40mg/L NAA+0.40mg/L IBA,生根率达100%,移栽成活率达80%.[结论]初步确定了绿玉树茎段组织培养的生长条件.%[ Objective ] The aim was to study the conditions of tissue culture regeneration seedling by using the stem segments of Euphorbia tirucalli and determine the optimum culture condition of each culture stage ,so as to provide references for the factory production and relative study of tissue culture seedling of E. tirucalli. [ Method] Taking the stem segments of E. tirucalli as explants,the effects of various mediums on germination rate,multiplication coefficient and rooting rate were studied. [ Result] The optimum induction medium of germination culture was 1/2MS +0.02 mg/L NAA + 1.00 mg/L 6-BA, with differentiation rate of 89.7％ ;the best subculture medium was 1/2MS + 0.02 mg/L NAA + 0.60 mg/L 6-BA + 3.00 mg/L AD, with multiplication coefficient of 5.70 ;the optimum rooting culture medium was 1/2MS + 0. 40 mg/L NAA + 0. 40 mg/L IBA ,with rooting rate of 100％ and transplanting survival rate of 80％. [ Conclusion] The tissue culture conditions of stem segments of E. tirucalli were determined primarily.
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