[Objective] The research aimed to study separating and purifying a-galactosidase from the germinating coffee beans. [Method] By using the separation and purification technologies, such as the ammonium sulfate precipitation, DEAE-Sepharose Fast Flow anion-exchange chromatograph and Sephacryl S-200-HR gel filtration chromatograph, α-galactosidase was separated and purified from the germinating coffee beans. SDA-PAGE was used to analyze the purity and molecular weight of enzyme. [ Result] After three steps separation and purification, the specific activity of germinating coffee beans a-galactosidase reached 191.50 nkat/mg,and the purification times was 23.18. Compared with the green coffee beans a-galactosidase, the specific activity of germinating coffee beans a-galactosidase was higher,but the productive ratio and total extraction ratio were lower. SDS-PAGE analysis result showed that the enzyme had the single band, and the molecular weight was 3 8805 D. [ Conclusion ] The improvements of germinating coffee beans a-galactosidase yield and total extraction ratio needed the further research.%[目的]研究木立芦荟中黄酮类化合物的微波法提取工艺.[方法]以黄酮类化合物提取率为指标,采用单因素试验和正交试验相结合的方法,对木立芦荟中黄酮类化合物的微波法提取工艺进行优化.[结果]微波法提取黄酮类化合物的最佳工艺条件为料液比1:50,乙醇浓度80%,微波功率560 W,微波处理时间为40s.在此条件下,测得的提取率为5.604%.[结论]与乙醇浸提法相比,微波法具有提取时间短、乙醇用量低、提取效率高的优点,是芦荟黄酮类化合物的理想提取方法.
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