荧光茧色判性家蚕黄酮合酶I(BmFNS I)基因在家蚕组织内的表达差异

摘要

采用荧光定量PCR分别检测不同品种荧光判性家蚕雌、雄家蚕的中肠、丝腺和脂肪体中的黄酮合酶I(BmFNS I)基因的表达.结果表明,家蚕BmFNS I基因在各品种荧光判性家蚕中肠中的相对表达量最高,其次是脂肪体,在丝腺中的表达量较低,说明BmFNSI基因在对家蚕食物桑叶内的黄酮类化合物的合成代谢中起重要作用;但是在各品种荧光判性家蚕的雌、雄中肠中的表达并无明显差异,说明荧光判性家蚕雌、雄蚕在对桑叶内的黄酮类化合物的合成代谢途径上不存在差异,至少在FNS I基因所调节的黄酮类化合物的合成代谢上不存在差异.%Expression differences of Flavonoid synthase I ( BmFNS I) gene in the midgut, silk gland and fat body of the male and female Bombyx mori with sex-identified fluorescent cocoon color was detected by fluorescence quantitative PCR. And the result showed that the relative expression level BmFNS I gene in the midgut of the silkwormn was the highest, followed by that in fat body, and the expression level in silk gland was the lowest, indicating that BmFNS I gene played an important role in flavonoids anabolism in leaves of mulberry, which was the food of silkworm. However, there was no significant difference in the BmFNS I gene expression level in the midgut between male and female Bombyx mori varieties with sex-identified fluorescent cocoon color, suggesting that there was no difference in anabolism of flavonoids in leaves between the male and female Bombyx mori with sex-identified fluorescent cocoon color, at least no difference in anabolism of flavonoids regula ted by flavonoid synthase I gene.