欧盟鱼病水平测试样品中DNA病毒检测

摘要

[ Objective ] By participating the European Union inter-laboratory proficiency test for fish diseases, the detectability of the laboratory was evaluated and the master of the detection method was improved. [ Method] Ten proficiency test samples were detected by virus isolation and identification according to the instruction of the proficiency test. [Result] The PTU was epizootic haematopoietic necrosis virus(EHNV) , the PTM was European catfish virus(ECV) , the PTVI and PTX were both koi herpesvirus disease virus( KHDV) , the PTK was epizootic ul-cerative syndrome( EUS). [ Conclusion] Four types of DNA virus were detected positive from the proficiency test samples, which was consistent with the final results.%[目的]通过参加欧盟鱼病水平测试,评价试验室检测能力,加强对检测方法的掌握.[方法]按照水平测试说明的方法进行病毒分离鉴定,测试的10个样品在病毒分离后用PCR方法检测DNA病毒.[结果]PTⅡ检测为流行性造血器官坏死病毒(Epizootic haematopoietic necrosis virus,EHNV),PTⅢ为欧洲鮰病毒(European catfish virus,ECV),PTⅧ和PTX为锦鲤疱疹病毒(Koi herpesvirus disease virus,KHDV),PTⅨ为流行性溃疡综合征(Epizootic ulcerative syndrome,EUS).[结论]该实验室检出了2011年欧盟鱼病水平测试样品中的4种DNA病毒,与最后公布的测试结果完全一致.