络石内生真菌Myrothecium roridum(IFB-E009)次生代谢产物的研究

摘要

该试验从络石(Trachelospermum jasminoides)内生的露湿漆斑菌属真菌Myrothecium roridum(IFB-E009)液体发酵产物中分离并鉴定了8个化合物,其中化合物1～4为单端孢霉烯类化合物,5～6为酰胺类化合物,7为环二肽化合物,8为尿嘧啶.活性测试表明,化合物1、4有细胞毒活性,对SW1116(人结肠癌细胞株)具有抑制作用,IC50分别为25.68和38.93 μmol/L,而对照药阿霉素的IC50为5.31μmol/L.该研究可为开发利用药用植物内生菌提供理论和实践依据.%Eight compounds were isolated and identified from liquid fermentation products of Myrothecium roridum ( IFB-E009 ) in Trachelospermum jasminoides, 1 -4 were trichotheeenes compounds, 5-6 were amides compounds, 7 was cyclic peptide, and 8 was uracil. As indicated by activity test, compound 1 , 4 showed activities to the human colon cancer cells SW1116. IC50 were 25.68 and 38.93 μmol/L, while the IC50 of adriamycin was 5.31 μmol/L. The study would provide theory and practice basis for the development and utilization of endophytes in pharmaceutical plants.