高效液相色谱法测定忍冬藤中木犀草素的含量

摘要

[目的]测定忍冬藤中木犀草素的含量.[方法]采用高效液相色谱法测定,色谱柱为Agilent ZORBAX SB-C1(1)(4.6 mm ×250 mm,5μm)；流动相为无水甲醇-浓度0.3％磷酸水溶液(47:53,V/V)；流速为1 ml/min;柱温为30℃；检测波长为为350 nm.[结果]木犀草素的线性范围为0.5～2.5 μg/ml,相关系数R=0.999 6,平均回收率为100.71％,RSD为1.9％；3批药材中木犀草素的平均含量为24.05μg/g.[结论]该方法简单、快速、可靠,适用于忍冬藤药材的质量控制.%[Objective] To determine the luteolin content in CAULIS LONICERAE. [Method] The HPLC method was performed on an Agilent ZORBAX SB- C18(4.6 mm ×250 mm,5 μm) using an isocratic elution with methanol-water containing 0. 3% phosphoric acid (47: 53,V/V) as the mobile phase at a flow rate of 1 ml/min, the column temperature was 30 ℃ and the detection wavelength was set at 350 nm. [ Result ] The linear rangs and regression equation of luteolin was 0.5 -2. 5 μg/ml with R =0.999 6. The average recovery was 100.71% ,RSD = 1.9%. The average luteolin content in the three batches of medicine was 24.05 μg/g. [ Conclusion ] This method is simple, rapid and reliable for the quality control of CAULIS LONICERAE.