中度嗜盐菌Halomonas sp.STSY-3的耐盐特性研究

摘要

[ Objective ] The study aimed to provide the method and parameter guidance for the screening of the engineering halophilic bacteria and also provide the necessary theoretical basis for the selecting, problem diagnosis and process optimization of the engineering bacterium during the biochemical treatment process of high salt wastewater through the study on salt-tolerant features of a moderately halophilic bacterium Halomonas sp. STSY-3. [ Method] With the moderately halophilic bacterium Halomonas sp. STSY-3 as the tested material, the effects of salinity , inoculation amount, anions and cations, osmotic pressure impact on the growth feature of this bacteria were determined by using the spectrophotometry. [Result] Halomonas sp. STSY-3 had a strong adaptability to the changes of salinity, the salinity range for the growth of this bacteria was 1 % - 11 % (NaCl, W/V) and the optimum salinity for the growth was 7% ( NaCl, W/V ). Under a series of NaCl concn. , the increasing of inoculation amount could effectively enhance the growth of this bacteria at different salinity. Among 12 kinds of anions and cations, only Cl" and Na+ were the optimum ions for its growth and the bacteria also had stronger tolerance to the SO42- . [ Conclusion ] The study provided the basic data of the biochemical treatment of high-salt wastewater and the determination method for the salt-tolerant features.%[目的]通过对中度嗜盐菌Halomonas sp.STSY-3的耐盐特性研究,为工程嗜盐菌的筛选提供方法和参数指导,同时为高盐废水的生化处理过程中工程菌的选用、问题诊断和工艺优化等提供必要的理论基础.[方法]以中度嗜盐菌Halomonas sp.STSY-3试验材料,利用吸光光度法测定盐度、接种量、阴阳离子和渗透冲击对该菌株生长特性的影响.[结果] Halomonas sp.STSY-3具有较强适应盐度变化的能力,菌株生长的盐度范围为1％～ 11％ (NaCl,W/V),最适生长盐度为7％(NaCl,W/V)；在系列NaCl浓度下,菌液接种量的增加可以有效地加强菌株在不同盐度下的生长；在12种不同的阴、阳离子中,仅有Cl-和Na+最适宜其生长,该菌株对SO42-也具有较强的耐受能力.[结论]该研究为高盐废水的生化处理提供了基础数据和耐盐特性测定方法.