人参皂甙Rbl对大鼠神经细胞表达蛋白质组的影响

摘要

[ Objective ] This study aimed to explore the effect of ginsenoside Rbl on expressive proteome of rat neurons by technologies of proteomics, bioinformatics and MS peptide fingerprinting. [ Method ] Rat neurons were cultured conventionally and randomly separated into two groups. The experimental group was treated with 5 fig /ml of Rbl for 20 min, while control group was added with the same amount of medium. After cell lysis, the whole-cell protein was extracted. Two-dimensional electrophoresis (2-DE) was used to separate the extracts. Differential expression of proteome between the two groups was analyzed by using ImageMaster 2D Platinum v5.0 software and the two protein spots expressed differently were selected for differential identification with MALDI-TOF-MS. [ Result ] Based on the matching and comparative analysis of the protein spots, 418 protein spots were detected in experimental group, including 226 protein spots with differently expressive levels; according to the mass spectrometry, the two ginsenoside Rbl-related and differentially-expressed protein spots were identified as cytochrome P-450 and phosducin-like protein, and both of them were phosphorylated proteins. [ Conclusion] This study showed that the functions of those I-dentified proteins were involved in signal transduction, suggesting that the effect of ginsenoside Rbl on expressive proteome of rat neurons might be related to the corresponding signal transduction networks.%[目的]研究人参皂甙Rbl对大鼠神经细胞表达蛋白质组的影响.[方法]常规培养大鼠神经细胞,将其随机分为2组,试验组加入5μg/ ml Rbl,对照组加入等量的培养基,药物作用20min,裂解细胞,提取全细胞蛋白.双向电泳(2- DE)分离提取物,用ImageMaster 2D Platinum v5.0软件进行差异表达蛋白质组分析,基质辅助激光解吸附离子化飞行时间质谱(MALDI - TOF - MS)鉴定差异表达蛋白质.[结果]通过对2 - DE图谱蛋白斑点的匹配及对比分析,试验组的2- DE图谱共检出蛋白斑点418个,其中226个为差异表达的蛋白斑点；经质谱鉴定,与Rhl作用相关的2个差异表达的蛋白斑点包括:细胞色素P -450、光导素样蛋白,它们均属于磷酸化蛋白质.[结论]该研究表明Rbl对大鼠神经细胞的作用极有可能是通过相应的细胞信号转导网络系统来实现的.